WebbFor researchers interested in testing our fluorescent WGA conjugates in their application, we also offer a Wheat Germ Agglutinin Stain Sampler Kit , which contains 1 mg quantities each of WGA conjugates of the Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine and Texas Red-X dyes. For ... Webb13 apr. 2024 · HER2 receptor aberrations are more common in breast DCIS premalignancy than in breast cancer. Here the authors identify a feedback circuit involving HER2 nucleocytoplasmic transport that may ...
Fluorescent imaging of endothelial glycocalyx layer with wheat …
Webb1 jan. 2011 · The results of this study show that fluorescent WGA lectin costaining is a cost-effective, fast, and convenient method for connective ... was injected into TBA … Webb1 dec. 2024 · RT-qPCR检测心肌梗死术后0、1、3和7 d心脏组 织中KLF15的mRNA表达变化。野生及KLF15基因敲除小鼠心肌梗死术后28 d,使用小动物心脏超声仪测定心功 能变化,Masson染色比较梗死纤维瘢痕,麦胚凝集素(wheat germ agglutinin,WGA)染色评价心肌细胞肥大情况。 kenneth wayne carswell texas
Measurement of Wheat Germ Agglutinin Binding with a …
WebbFluorescent wheat germ agglutinin (FWGA) is another tool that has been used for PG labelling. WGA is a protein found abundantly in wheat kernels. It has a high binding affinity for N -acetyl-glucosamine (GlcNAc), one of the essential components of PG glycan strands, and thus can be used to probe PG specifically. Webb16 aug. 2024 · Glycocalyx was labeled with AF 594-conjugated WGA (WGA-Alexa; Thermo Fisher Scientific; 100 μ L of 1 mg/mL solution in saline) injected i.v. 45 min before imaging. To enzymatically degrade the glycocalyx, we used hyaluronidase (Sigma-Aldrich type IV-S; 750–3,000 units per milligram; 5 mg in 150 μ L saline) injected into the … Webb19 nov. 2015 · We examined the affinity of the different lectins to epivascular regions under an intravital fluorescent microscope. Among seven different lectins we examined, FITC … kenneth wayne dockery brandon fl