WebSMART ( S witching M echanism a t the 5′ end of R NA T emplate) technology leverages the template-switching capability of certain reverse transcriptases (RTs) to capture full-length … During first-strand synthesis when priming with an oligo dT primer to capture polyadenylated transcripts, upon reaching the 5' end of the RNA template, the terminal transferase activity of the MMLV reverse transcriptase adds a few additional nucleotides (mostly deoxycytidine) to the 3' end of the newly … See more Composition:Chimeric DNA/RNA Oligo Ordering path: Order as a Custom RNA Oligo Typical length:~30 bases Scales: 100 nmol to large … See more Emerging high-throughput technologies such as RNA-seq have enabled functional dissection of complex transcriptomes with a minimal amount of RNA input. Quantification of … See more The simple version of a TS Oligo is a DNA oligo sequence that carries 3 riboguanosines (rGrGrG) at its 3' end [1]. The complementarity … See more Described originally in 2001, a strategy frequently referred to as “SMART” (switching mechanism at the 5' end of the RNA transcript) technology (Takara Bio USA, Inc) has shown … See more
Long-fragment, strand-specific PCR–cDNA libraries and …
WebSAGE Journals: Your gateway to world-class research journals Web24 Dec 2024 · Strand-switching (similar to 5’ rapid amplification of cDNA ends [RACE]) only needs one primer-binding site for the synthesis of full-length cDNA, which is … faculty x colin wilson
PCR-cDNA Sequencing Kit - Home - Nanopore Store
Web13 Nov 2014 · Illustration of the primers used to prepare RNA-seq libraries for high-throughput sequencing. (a) Generic oligonucleotide design, showing portions of each primer and corresponding function.(b) Sequences of the oligonucleotides used to generate Illumina-specific libraries.The RT and template-switching primers are shown at the top, and the … WebThe transcript strand is preserved (Fig. 1a). We observe a good fraction of the reference transcripts covered by individual reads up to ~5 kb (Fig. 1b). The presence of the reverse transcription and strand-switching primer sequences in the cDNA sequences allows us to select these full-length reads from the dataset (52.2% of total reads). WebTaking full-length poly-A+ RNA, complementary strand synthesis and strand switching are performed using kit-supplied oligonucleotides. The RNA template is then digested, and the second (complementary) strand is synthesised. Sequencing adapters supplied in the Direct cDNA Sequencing Kit are then ligated onto the dscDNA. faculty yearbook pages